Recombinant Factor C Endotoxin Detection

Bacteriophages instead of Horshoe Crabs: The innovative technology behind EndoLISA® Endotoxin Detection Assay is based on LPS-specific binding proteins derived from naturally occurring bacteriophages. This picture shows T4 bacteriophages infecting a live E. coli bacteria cell (source: Dr.Wanner Munich/Hyglos GmbH)

http://www.endotoxin-test.com/endotoxin-detection/

Endotoxin detection through Recombinant Factor C

The world’s first endotoxin detection system based on ELISA-technology.

In September 2011, Hyglos introduced EndoLISA® a recombinant factor C (rFC) endotoxin detection product - a new method for efficient and reliable determination of endotoxins (LPS) based on the proprietary phage ligand technology. An enormous advantage of Hyglos’ ELISA-based assay is the heterogenous microtiter plate based format (96-well microplate with 8-well strips). The LPS from the sample is specifically immobilized for detection while interfering ingredients such as proteins or beta glucans are removed by a washing step. This enables the Hyglos method to precisely determine the endotoxin without matrix effects, without dilution and with a broad pH tolerance. The detection reaction in EndoLISA® is based on Recombinant Factor C (rFC). In January 2013, Hyglos introduced EndoZyme® – a further assay based on Recombinant Factor C without the solid phase, being suitable for medical device and water testing.
True Innovation with Phage recombinant Technology and recombinant Horseshoe Crab Factor C
Highly specific and robust detection of endotoxin (LPS) helps you make confident in process control (IPC) and product release decisions:

 

• Selecting the ideal endotoxin (LPS) test method for your samples
• Removing inhibitory effects due to buffer and sample components
• Improving reproducibility of results
• Optimizing endotoxin recovery

Assays:

EndoZyme® rFC Assay

EndoZyme® !! NEW !! Homogeneous fluorescence microplate assay for the quantitative determination of endotoxins (lipopolysaccharides). EndoZyme® offers excellent LPS-specificity, a very high test sensitivity (0.005 EU/ml) as well as a broad measurement range (0-50 EU/ml). Characterized by an excellent lot-to-lot consistency (click picture  for product information)

 

EndoLISA® Hetereogeneous fluorescence microplate assay using a phage-derived capture molecule for the quantitative binding and determination of endotoxins (lipopolysaccharides). The world’s first endotoxin detection system based on ELISA-technology. A highly robust method with unprecedented salt and detergent tolerance. Developed and intended for complex sample matrices. (click picture for product information)http://www.endotoxin-test.com/endotoxin-detection/