A new study evaluates the endotoxin removal efficiency of four endotoxin removal resins from different suppliers, including EndoTrap® HD:
Reich et al. (Univ. Regensburg) studied endotoxin removal and sample recovery rates of four commercially available affinity chromatography resins.
To this end, BSA samples [1 mg/ml] in commonly used buffers (Borate, TRIS, MES and Acetate) containing 15,000 EU/ml E.coli 055:B5 LPS, were purified with each 1 ml of the different resins in gravity flow columns according to the manufacturers’ instructions.
EndoTrap® HD supplied by Hyglos was shown to be the most effective resin for removing endotoxins in comparison to the other resins tested. Furthermore, the purification with the other resins resulted in higher sample loss.
Results
Table 1. BSA samples [1 ml of 1 mg/ml] in 20mM Borate buffer,
150 mM NaCl, 0.1 mM CaCl2, pH 9, containing 15,000 EU/ml
were purified with the indicated resins
| Column1 | Column2 | |
| Endotoxin Removal |
Sample Recovery | |
| EndoTrap® HD (Hyglos) | >99,99% | 100% |
| Pierce High Capacity Resin (Thermo Fisher) | 51.71% | 98% |
| EndoBind-R™ (BioDtech, Inc.) | >99,99% | 87% |
| ToxinEraser™ (GenScript) | >99,99% | 84% |
Table 2. BSA samples [1 ml of 1 mg/ml] in 20 mM TRIS,
150 mM NaCl, 0.1 mM CaCl2 pH 7.4, containing
15,000 EU/ml were purified with the indicated resins.
| Column1 | Column2 | |
| Endotoxin Removal |
Sample Recovery | |
| EndoTrap® HD (Hyglos) | >99,99% | 99% |
| Pierce High Capacity Resin (Thermo Fisher) | >99,99% | 88% |
| EndoBind-R™ (BioDtech, Inc.) | >99,99% | 15% |
| ToxinEraser™ (GenScript) | >99,99% | 69% |
Table 3. BSA samples [1 ml of 1 mg/ml] in 20mM MES,
150 mM NaCl, 0.1 mM CaCl2, pH 6, containing
15,000 EU/ml were purified with the indicated resins.
| Column1 | Column2 | |
| Endotoxin Removal |
Sample Recovery | |
| EndoTrap® HD (Hyglos) | >99,99% | 97% |
| Pierce High Capacity Resin (Thermo Fisher) | 99.55% | 82% |
| EndoBind-R™ (BioDtech, Inc.) | 99.96% | 69% |
| ToxinEraser™ (GenScript) | >99,99% | 90% |
Table 4. BSA samples [1 ml of 1 mg/ml] in 20mM sodium
acetate, 150 mM NaCl, 0.1 mM CaCl2, pH 5, containing
15,000 EU/ml were purified with the indicated resins.
| Column1 | Column2 | |
| Endotoxin Removal |
Sample Recovery | |
| EndoTrap® HD (Hyglos) | >99,99% | 97% |
| Pierce High Capacity Resin (Thermo Fisher) | 99.97% | 98% |
| EndoBind-R™ (BioDtech, Inc.) | 99.82% | 96% |
| ToxinEraser™ (GenScript) | 99.96% | 96% |
EndoTrap® HD at a glance:
•Suitable for a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, DNA, buffers and cell extracts
•Exceptional endotoxin binding capacity of 5,000,000 EU/ml
•Highly efficient and specific endotoxin removal and sample recovery rates
•Multiple reusability of more than 10 times
•Reaching a LPS concentration of under 0.1 EU/ml
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